Invitrogen™ Dectin 1 (soluble) Monoclonal Antibody (2A11)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA516478

Catalog No. PIMA516478

Description

The epitope recognized by 2A11 is sensitive to H2O2 treatment, therefore quenching with 0.3% H2O2 should be performed after incubation with the primary antibody. The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug. Rat anti Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.

CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.

Specifications

• Antigen: Dectin 1 (soluble)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q6QLQ4
• Gene Symbols: Clec7a
• Immunogen: Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation
• Clone: 2A11
• Conjugate: Unconjugated
• Gene: Clec7a
• Gene Alias: Beta-glucan receptor; beta-GR; BGR; CANDF4; CD369; CLEC7A; Clec7b; CLECSF12; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 12; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; DC-associated C-type lectin 1; DECTIN1; dectin-1; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; lectin-like receptor 1; RGD1565140; SCARE2; UNQ539/PRO1082
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 56644
• Content And Storage: 4°C, do not freeze
• Form: Liquid