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PeproTech DEFB104A Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No. 502718552
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Catalog No. Quantity
50-271-8552 500 μg
500P268BT25 25 μg
500P268BT50 50 μg
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Catalog No. 50-271-8552 Supplier PeproTech Supplier No. 500P268BT500UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Humn BD-4. Anti-Human BD-4-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hBD-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-4 (500-P268) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-4. Western Blot: To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P268BT-1MG will be provided as 2 x 500 μg

Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104A, represents the more centromeric copy.
TRUSTED_SUSTAINABILITY

Specifications

Antigen DEFB104A
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Biotin
Formulation PBS with no preservative
Gene DEFB104A
Gene Accession No. Q8WTQ1
Gene Alias BD-4; Beta-defensin 104; Beta-defensin 4; DEFB104; DEFB104A; DEFB104B; DEFB4; DEFB-4; defensin beta 104A; Defensin, beta 104; defensin, beta 104A; defensin, beta 4; hBD-4
Gene Symbols DEFB104A
Host Species Rabbit
Immunogen E.coli-derived Recombinant Human BD-4.
Purification Method Antigen affinity chromatography
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 140596
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
Isotype IgG
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