Invitrogen™ DEFB104A Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P268BT500UG

Catalog No. 50-271-8552

Description

AA Sequence of recombinant protein: EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Humn BD-4. Anti-Human BD-4-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hBD-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-4 (500-P268) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-4. Western Blot: To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P268BT-1MG will be provided as 2 x 500 μg

Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104A, represents the more centromeric copy.

Specifications

• Antigen: DEFB104A
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: DEFB104A
• Gene Alias: BD-4; Beta-defensin 104; Beta-defensin 4; DEFB104; DEFB104A; DEFB104B; DEFB4; DEFB-4; defensin beta 104A; Defensin, beta 104; defensin, beta 104A; defensin, beta 4; hBD-4
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 140596
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q8WTQ1
• Gene Symbols: DEFB104A
• Immunogen: E.coli-derived Recombinant Human BD-4.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG