Invitrogen™ Dendritic Cell Marker DCIR2 Monoclonal Antibody (33D1), PerCP-eFluor™ 710, eBioscience™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
46-588-480	Invitrogen™ 46588480	25 μg

Description

Description: The 33D1 monoclonal antibody reacts with a mouse dendritic cell (DC)-specific surface marker, DCIR2. The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii. Applications Reported: This 33D1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 33D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please ...

The nature and biological activity of DCIR2 is unknown. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

Specifications

• Antigen: Dendritic Cell Marker DCIR2
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: 0
• Gene ID (Entrez): 474145
• Primary or Secondary: Primary
• Purification Method: Affinity Chromatography

• Clone: 33D1
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2b κ
• Target Species: Mouse
• Gene: Clec4a4
• Gene Alias: Clec4a4; C-type lectin domain family 4, member a4; DC Marker; Dcir2; dendritic cell inhibitory receptor 2
• Gene Symbols: Clec4a4
• Product Type: Antibody