Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Buffers and Standards
  4. General Chemistry Solutions
  5. J63135AEPM

Denhardt's Solution, 50X, PROMO, Thermo Scientific Chemicals

Catalog No. J63135AEPM
Click to view available options
Quantity:
100 mL

Description

Denhardt's solution is a mixture of blocking agents used in membrane-based hybridization protocols.

General Description

  • Formulated in 1966, Denhardt's solution is a combination of high-molecular weight polymers used to prevent non-specific binding of nucleic acid probes to membranes and filters
  • Denhardt's solution contains 1% Ficoll (type 400), 1% polyvinylpyrrolidone, and 1% bovine serum albumin

Application

  • Denhardt's solution is used in Northern and Southern blotting to prevent non-specific binding of detection reagents to membranes
  • It can be used along with gelatin-chrome alum in in situ hybridization assays to maintain chromosome integrity
  • It has been used to facilitate quantitative DNA hybridization on nylon membranes
TRUSTED_SUSTAINABILITY

Specifications

Chemical Name or Material Denhardt's solution
MDL Number MFCD00717549
Concentration 50X
Solubility Information Fully miscible.
Quantity 100 mL
Physical Form Liquid

RUO - Research Use Only

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.