Desmin Monoclonal Antibody (DE-U-10), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-2815	Invitrogen™ 14974780	25 μg
50-112-2816	Invitrogen™ 14974782	100 μg

Description

Description: The monoclonal antibody DE-U-10 recognizes desmin. Desmin is the protein subunit of class-III intermediate filaments. Desmin is found predomitly in skeletal, cardiac, and smooth muscle. Desmin plays a role in muscle cell development and differentiation, muscle cell architecture and structure, and mitochondrial function. Desmin forms scaffolds around the Z-disk of sarcomeres in muscle cells. Desmin knockout mice exhibit defects in skeletal, smooth, and cardiac muscle and have impaired mitochondrial function. Mutations in Desmin result in conditions such as desmin-related myopathy (DRM), cardiomyopathy dilated type 1I (CMD1I), and neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome). The DE-U-10 antibody recognizes human, mouse, rat, feline, bovine, goat, hamster, sheep, porcine, rabbit, and chicken desmin. The DE-U-10 antibody will also label tumors derived from muscle tissue, such as leiomyomas and rhabdomyosarcomas. Applications Reported: This DE-U-10 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This DE-U-10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 5 μg/mL.

Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

Specifications

• Antigen: Desmin
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: CMD1I; CSM1; CSM2; DES; Desmin; desmin-like protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; I79_018932; intermediate filament protein; LGMD2R; MFM1; muscle-specific intermediate filament desmin; muscle-specific intermediate filament protein; mutant desmin p.K241E; SCPNK; similar to desmin
• Gene Symbols: DES
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: DES

• Clone: DE-U-10
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Rat, Rabbit, Bovine, Sheep, Hamster, Pig, Feline, Chicken, Goat
• Gene Accession No.: O62654, P02540, P02542, P17661, P31001, P48675
• Gene ID (Entrez): 100328890, 100764990, 101097010, 101117308, 102180712, 13346, 1674, 280765, 395906, 396725, 64362
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.