Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Columns, 1 mL PROMO

Description

Bind and extract endotoxins (lipopolysaccharides or LPS) from antibody or protein samples using bulk resin or columns of immobilized Polymixin B ligand.

Detoxi-Gel Resin is a beaded agarose support onto which polymixin B has been immobilized. This ligand binds to the lipid A portion of lipopolysaccharides (LPS), which are the dominant form of endotoxins that affect biological samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be added to cells for various treatment experiments. Detoxi-Gel Resin provides a quick method for removal of endotoxins from samples. It is available in convenient pre-packed columns and two sizes of slurry for use in small-scale or large-scale processes.

Highlights:

• Affinity-based – uses immobilized polymixin B to remove endotoxins by binding to their lipid A domains

• High capacity – 1 mL of Detoxi-Gel Resin removes at least 9995 endotoxin units from a 5 mL sample containing 10,000 EU of lipopolysaccharide (LPS)

• Reusable – no loss in binding capacity even after 10 regenerations; requires activation and regeneration with 1% sodium deoxycholate (Part No. 89904)

• Testing required – protein recovery is dependent upon protein type and concentration; empirical testing is required to validate the method for particular protein samples

• Versatile – remove endotoxin from protein solutions, cell culture media and pharmacological components

Requires:

Sodium deoxycholate (Part No. 89904) for resin activation and regeneration.

Specifications

• Capacity (Metric): 1 mL
• Content And Storage: Store at 4°C.
• Form: Liquid
• Product Line: Detoxi-Gel™
• Quantity: 5 Columns
• Target: Endotoxin Removal
• Type: Endotoxin Removing Column
• Stationary Phase: Polymixin B
• Format: Spin Column
• Column Type: Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.