DFF45/DFFA Polyclonal Antibody, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc IHC00291T

Catalog No. 50-157-1838

Description

The recommended shelf life for this product is 1 year from date of receipt. Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Chimpanzee and Northern white-cheeked gibbon

ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.

Specifications

• Antigen: DFF45/DFFA
• Classification: Polyclonal
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene Accession No.: O00273
• Gene Symbols: DFFA
• Immunogen: Between 271 and 331
• Quantity: 10 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunofluorescence, Immunohistochemistry
• Conjugate: Unconjugated
• Gene: DFFA
• Gene Alias: DFF, ICAD, DNA fragmentation factor 45 kDa subunit, DFF-45, Inhibitor of CAD, ICAD
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1676
• Content And Storage: 4° C
• Form: Liquid