Tocris Bioscience™ DFHBI 1T

Description

Mimic of GFP fluorophore for imaging RNA in living cells

DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers. DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

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Specifications

• Chemical Name or Material: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
• CAS: 1539318-36-9
• Quantity: 10 mg
• Target: Light-up Aptamers
• Molecular Formula: C₁₃H₉F₅N₂O₂
• Formula Weight: 320.21
• Purity: >98%