Invitrogen™ DGKI Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5106463

Catalog No. PIPA5106463

Description

Antibody detects endogenous levels of total DGKI.

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and - γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-ι (130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-epsilon (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-Ω (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.

Specifications

• Antigen: DGKI
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DGKI
• Gene Alias: C130010K08Rik; DAG kinase iota; DGKI; DGK-iota; Diacylglycerol kinase iota; diacylglycerol kinase iota-3; diacylglycerol kinase, iota; Diglyceride kinase; diglyceride kinase iota; EC 2.7.1.107; rDGKi-3
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 320127, 688705, 9162
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O75912
• Gene Symbols: DGKI
• Immunogen: A synthesized peptide derived from human DGKI(Accession O75912), corresponding to amino acid residues A1003-Y1053.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG