Invitrogen™ DHFR Monoclonal Antibody (3C8)

Catalog No.	Quantity
PIMA549170	100 μg

Catalog No. PIMA549170 Supplier Invitrogen™ Supplier No. MA549170

Description

Mouse Monoclonal Antibody

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human DHFR shares 90% amino acid (aa)sequence identity with both mouse and rat DHFR. Positive Control - WB: human K562 whole cell, human SGC-7901 whole cell, human Hela whole cell, human SW620 whole cell, rat kidney tissue. IHC: human rectal cancer tissue, rat gaster tissue. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Specifications

• Antigen: DHFR
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 3C8
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene: DHFR
• Gene Accession No.: P00374, Q920D2
• Gene Alias: 8430436I03Rik; AA607882; AI662710; AW555094; Dhfr; Dhfr1; DHFRP1; Dihydrofolate reductase; Dihydrofolate reductase 1 (active); DYR
• Gene Symbols: DHFR
• Host Species: Mouse
• Immunogen: E.coli-derived human DHFR recombinant protein (Position: V2-D187).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1719, 24312
• Target Species: Human, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG2b