Invitrogen™ DHFR Monoclonal Antibody (3C8)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA549170

Catalog No. PIMA549170

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Human DHFR shares 90% amino acid (aa)sequence identity with both mouse and rat DHFR. Positive Control - WB: human K562 whole cell, human SGC-7901 whole cell, human Hela whole cell, human SW620 whole cell, rat kidney tissue. IHC: human rectal cancer tissue, rat gaster tissue. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, and is required for the synthesis of purines, thymidylic acid and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Specifications

• Antigen: DHFR
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4 mg trehalose and 0.05 mg sodium azide
• Gene Accession No.: P00374, Q920D2
• Gene Symbols: DHFR
• Immunogen: E.coli-derived human DHFR recombinant protein (Position: V2-D187).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: 3C8
• Conjugate: Unconjugated
• Gene: DHFR
• Gene Alias: 8430436I03Rik; AA607882; AI662710; AW555094; Dhfr; Dhfr1; DHFRP1; Dihydrofolate reductase; Dihydrofolate reductase 1 (active); DYR
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1719, 24312
• Content And Storage: -20°C
• Form: Lyophilized