R&D Systems™ Diaminobenzidine (DAB)

Description

R&D Systems™ Diaminobenzidine (DAB) Solution is a component of the reagent kit that is used for in situ detection of apoptosis in tissue sections and cells.

• Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Do not use past expiration date.
• Thaw at 37°C for 30 min.
• If not using the full bottle, store the remaining stock solution back at -20°C.
• It is not recommended to freeze thaw DAB stock solution more than three times.
• If required, aliquot in smaller volume and store at -20°C protected from light.
• It is recommended that 6mL aliquots of fresh 30% hydrogen peroxide are made and stored at 4°C.
• For each labeling procedure use a fresh 30% hydrogen peroxide aliquot then discard any remaining solution.
• Do not place on ice after thawing, otherwise the solution will precipitate.
• Prepare the solution no more than 30 minutes before use.
• Use 50mL of DAB Solution to process 1 to 10 samples.

Specifications

• Quantity: 3.75 mL
• Content And Storage: Store the unopened product at -70° C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.