Invitrogen™ Dicer Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578446

Catalog No. PIPA578446

Description

Positive Control: Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, 293T, A431, HeLa, HepG2, MCF-7, SW480 Predicted Reactivity: Zebrafish (93%), Xenopus laevis (92%), Pig (99%), Chicken (96%), Rhesus Monkey (100%), Bovine (99%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.

Specifications

• Antigen: Dicer
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DICER1
• Gene Alias: 1110006F08Rik; D12Ertd7e; DCR; DCR1; DCR-1; Dicer; dicer 1 ribonuclease III; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease III; dicer 1, ribonuclease type III; Dicer1; Dicer1, Dcr-1 homolog; Dicer1e; double-strand-specific ribonuclease mDCR-1; endoribonuclease Dicer; Helicase MOI; helicase with RNAse motif; helicase-moi; HERNA; K12H4.8-LIKE; KIAA0928; mKIAA0928; MNG1; RMSE2
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 192119, 23405, 299284
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.7 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q8R418, Q9UPY3
• Gene Symbols: DICER1
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Dicer. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov