Diethyl Pyrocarbonate, MP Biomedicals™

Catalog No.	Quantity
MP215090280	100 mL

Catalog No. MP215090280 Supplier MP Biomedicals, Inc Supplier No. 0215090280

Description

Used to inactivate RNase enzymes. Diethyl Pyrocarbonate, MP Biomedicals is sensitive to moisture and pH. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. It protects RNA against degradation and is effective

DEPC inactivates RNase in solution at about 0.1% (v/v). It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. DEPC is effective as a nuclease inhibitor with a variety of uses:

• Modification reagent for His and Tyr residues in proteins
• Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases
• Used to modify proteins and nucleic acids
• PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases
• Dot blot hybridization, to dilute total RNA isolated from different micro-organisms

Specifications

Chemical Identifiers

• CAS: 1609-47-8
• Molecular Formula: C6H10O5
• Molecular Weight (g/mol): 162.14
• MDL Number: MFCD00009106
• InChI Key: FFYPMLJYZAEMQB-UHFFFAOYSA-N
• Synonym: diethyl pyrocarbonate, diethyl dicarbonate, depc, diethyl oxydiformate, baycovin, diethylpyrocarbonate, ethyl pyrocarbonate, dicarbonic acid, diethyl ester, dicarbonic acid diethyl ester, diethyl pyrocarbonic acid
• PubChem CID: 3051
• ChEBI: CHEBI:59051
• IUPAC Name: ethoxycarbonyl ethyl carbonate
• SMILES: CCOC(=O)OC(=O)OCC

• Density: 1.101g/mL at 25°C (Lit.)
• Boiling Point: 93°C to 94°C (18 mmHg)(literature)
• Refractive Index: n20/D 1.398 (Lit.)
• Quantity: 100 mL
• Solubility Information: Soluble in alcohols, esters, ketones, hydrocarbons. A 50% w/w solution in ethanol may be prepared. Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2.
• Formula Weight: 162.10
• Concentration or Composition (by Analyte or Components): Working Concentration: For Treatment of water to inhibit Rnase: Add 1mL DEPC to 1L water. Let sit overnight. Autoclave.
• Physical Form: Liquid

Safety and Handling

Recommended Storage

Store at 4°C, under nitrogen.