Diethyl Pyrocarbonate, MP Biomedicals™

Description

Used to inactivate RNase enzymes. Diethyl Pyrocarbonate, MP Biomedicals is sensitive to moisture and pH. DEPC is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. It protects RNA against degradation and is effective

DEPC inactivates RNase in solution at about 0.1% (v/v). It inhibits the ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. DEPC is effective as a nuclease inhibitor with a variety of uses:

• Modification reagent for His and Tyr residues in proteins
• Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases
• Used to modify proteins and nucleic acids
• PCR reaction for treating deionized water, which reduces the risk of RNA being degraded by RNases
• Dot blot hybridization, to dilute total RNA isolated from different micro-organisms

Specifications

Chemical Identifiers

• CAS: 1609-47-8
• Molecular Formula: C6H10O5
• Molecular Weight (g/mol): 162.14
• MDL Number: MFCD00009106
• InChI Key: FFYPMLJYZAEMQB-UHFFFAOYSA-N
• Synonym: diethyl pyrocarbonate, diethyl dicarbonate, depc, diethyl oxydiformate, baycovin, diethylpyrocarbonate, ethyl pyrocarbonate, dicarbonic acid, diethyl ester, dicarbonic acid diethyl ester, diethyl pyrocarbonic acid
• PubChem CID: 3051
• ChEBI: CHEBI:59051
• IUPAC Name: ethoxycarbonyl ethyl carbonate
• SMILES: CCOC(=O)OC(=O)OCC

• Density: 1.101g/mL at 25°C (Lit.)
• Boiling Point: 93°C to 94°C (18 mmHg)(literature)
• Refractive Index: n20/D 1.398 (Lit.)
• Quantity: 100 mL
• Solubility Information: Soluble in alcohols, esters, ketones, hydrocarbons. A 50% w/w solution in ethanol may be prepared. Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2.
• Formula Weight: 162.10
• Concentration or Composition (by Analyte or Components): Working Concentration: For Treatment of water to inhibit Rnase: Add 1mL DEPC to 1L water. Let sit overnight. Autoclave.
• Physical Form: Liquid

Safety and Handling

Recommended Storage

Store at 4°C, under nitrogen.