Invitrogen™ Diphtheria Toxin Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA17212

Catalog No. PA17212

Description

PA1-7212 detects diphtheria toxin. PA1-7212 has been successfully used in ELISA procedures. The PA1-7212 immunogen is purified diphtheria toxoid. For longer term storage, aliquot and freeze at -20ºC.

Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.

Specifications

• Antigen: Diphtheria Toxin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: DT
• Immunogen: Purified diphtheria toxoid.
• Quantity: 1 mL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA
• Concentration: 5.0 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.2
• Host Species: Goat
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov