Invitrogen™ Diphtheria Toxin Polyclonal Antibody, HRP

Catalog No.	Quantity
PA173138	1 mL

Catalog No. PA173138 Supplier Invitrogen™ Supplier No. PA173138

Description

Goat Polyclonal Antibody

The PA1-73138 antibody reacts with Diphtheria toxin. PA1-73138 has been successfully used in ELISA applications. The PA1-73138 antibody was raised against purified diphtheria toxoid.

Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B.

Specifications

• Antigen: Diphtheria Toxin
• Applications: ELISA
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: HRP
• Formulation: PBS with 10mg/mL BSA and 0.002% thimerosal
• Gene Alias: DT
• Host Species: Goat
• Immunogen: Purified diphtheria toxoid.
• Purification Method: IgG fraction
• Quantity: 1 mL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Bacteria
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

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