MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 HPLC Column, 3 μm Particle Size

Description

Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC.

Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC. Long-chain phases, like C8 or C18, are often too hydrophobic for proteins and can cause excessively long retention time or even irreversible binding to the column. For this reason short-chain phases, typically C3 or C4, are often used for RP-HPLC of proteins. However, these short-chain phases are susceptible to hydrolysis, resulting in short column lifetime, especially at low pH. The Discovery™ BIO Wide Pore C5 gives elution order similar to a conventional C4, yet has enhanced pH stability for longer column lifetime. Generally, higher efficiency separations are achievable on the Discovery™ BIO Wide Pore C5 because of the improvements we have made to the silica and bonded-phase chemistry.

Specifications

• Diameter (Metric): 1 mm
• Length (Metric): 5 cm
• Particle Size: 3 μm
• Pore Size: 300 Å
• Surface Area: 100 m2/g
• Stationary Phase: C5 (pentyl)
• Product Line: Discovery
• Matrix: C5 (pentyl) Phase/Silica Particle Platform
• Endcapped: Yes
• Phase: Reversed Phase
• Technique: Wide Pore HPLC

Discovery is a registered trademark of Sigma-Aldrich Co. LLC