Invitrogen™ DKK3 Recombinant Rabbit Monoclonal Antibody (019)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA531106

Catalog No. PIMA531106

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Mouse Dkk3.

DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

Specifications

• Antigen: DKK3
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q9QUN9
• Gene Symbols: DKK3
• Immunogen: Recombinant Mouse Dkk3 protein (Met1-Ile349).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA
• Clone: 019
• Conjugate: Unconjugated
• Gene: DKK3
• Gene Alias: AW061014; C87148; Dickkopf; dickkopf 3; dickkopf 3 homolog; Dickkopf gene 3; dickkopf homolog 3; dickkopf homolog 3 (Xenopus laevis); dickkopf WNT signaling pathway inhibitor 3; Dickkopf3; dickkopf-3; Dickkopf-related protein 3; DKK 3; Dkk3; dkk-3; hDkk 3; hDkk-3; hhDkk3; mDkk-3; p29; regulated in glioma; Reic; RIG; RIG-like 5-6; RIG-like 7-1; UNQ258/PRO295; Xenopus
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 50781
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid