Invitrogen™ DLAT Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120279

Catalog No. PIPA5120279

Description

Positive test controls include: HeLa, MCF7, HepG2, K-562, Mouse brain, Rat brain. The target is usually found in the following locations: Mitochondrion matrix. Immunogen sequence: SLPPHQKVPL PSLSPTMQAG TIARWEKKEG DKINEGDLIA EVETDKATVG FESLEECYMA KILVAEGTRD VPIGAIICIT VGKPEDIEAF KNYTLDSSAA PTPQAAPAPT PAATASPPTP SAQAPGSSYP PHMQVLLPAL SPTMTMGTVQ RWEKKVGEKL SEGDLLAEIE TDKATIGFEV QEEG.

DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure.

Specifications

• Antigen: DLAT
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DLAT
• Gene Alias: 6332404G05Rik; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; anti DLAT; Dihydrolipoamide; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoamide S-acetyltransferase; dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex); dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; DLAT; DLTA; E2; E2 component of pyruvate dehydrogenase complex; EC 2.3.1.12; M2 antigen complex 70 kDa subunit; ODP2; PBC; PDC PDC-E2; PDCE2; PDC-E2; pyruvate dehydrogenase complex component E2
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1737, 235339, 81654
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1.31 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P08461, P10515, Q8BMF4
• Gene Symbols: DLAT
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 87-270 of human DLAT (NP_001922.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG