Invitrogen™ DMPO Monoclonal Antibody (N1664A), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545262

Catalog No. PIMA545262

Description

A 1:1,000 dilution of MA5-45262 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.

Specifications

• Antigen: DMPO
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS, 9.1mM sodium bicarbonate with 581mM DMSO, 123mM ethanolamine and no preservative; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry, Microarray
• Clone: N1664A
• Conjugate: FITC
• Gene Alias: DMPO nitrone adduct
• Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark
• Form: Liquid
• Target Molecule: Chemical

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov