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Invitrogen™ DMPO Monoclonal Antibody (N1664A), FITC

Catalog No. PIMA545262
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100 μg
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Catalog No. Quantity
PIMA545262 100 μg
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Catalog No. PIMA545262 Supplier Invitrogen™ Supplier No. MA545262
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Description

Mouse Monoclonal Antibody

A 1:1,000 dilution of MA5-45262 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.
TRUSTED_SUSTAINABILITY

Specifications

Antigen DMPO
Applications ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry, Microarray
Classification Monoclonal
Clone N1664A
Concentration 1 mg/mL
Conjugate FITC
Formulation PBS, 9.1mM sodium bicarbonate with 581mM DMSO, 123mM ethanolamine and no preservative; pH 7.4
Gene Alias DMPO nitrone adduct
Host Species Mouse
Immunogen 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
Purification Method Protein G
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage 4°C, store in dark
Product Type Antibody
Form Liquid
Isotype IgG1
Target Molecule Chemical
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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