Invitrogen™ DMPO Monoclonal Antibody (N1664A)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527550

Catalog No. PIMA527550

Description

A 1:1,000 dilution of MA5-27550 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.

The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.

Specifications

• Antigen: DMPO
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry, Microarray
• Clone: N1664A
• Conjugate: Unconjugated
• Gene Alias: DMPO nitrone adduct
• Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Form: Liquid
• Target Molecule: Chemical