DNA G-quadruplex (G4) Mouse anti-All, Clone: 1H6, MilliporeSigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABE1126

Catalog No. MABE1126MI

Description

This Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex .

Specifications

• Antigen: DNA G-quadruplex (G4)
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: All species
• Form: Purified

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA, Flow Cytometry
• Clone: 1H6
• Conjugate: Unconjugated
• Immunogen: KLH-conjugated G-quadruplex DNA derived from oligonucleotides with telomeric repeats.
• Quantity: 100 μg
• Research Discipline: Epigenetics and Nuclear Function
• Test Specificity: Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity, while exhibiting significantly lower affinity toward tetramolecular RNA structure or a triplex DNA structure, and little to no affinity toward either non-G4 ssDNA or ddDNA. However, clone 1H6 does not seem to bind the [AGGG(TTAGGG)3] unimolecular G4 structure, indicating a broad selectivity toward many, but not all, DNA G4 structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG2b κ