Invitrogen™ DNA Ligase IV Recombinant Rabbit Monoclonal Antibody (2T6J2)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA535109

Catalog No. PIMA535109

Description

Immunogen sequence: SRVGSGCTMK ELYDLGLKLA KYWKPFHRKA PPSSILCGTE KPEVYIEPCN SVIVQIKAAE IVPSDMYKTG CTLRFPRIEK IRDDKEWHEC MTLDDLEQLR GKASGKLASK HLYIGGDDEP QEKKRKAAPK MKKVIGIIEH LKAPNLTNVN KISNIFEDVE FCVMSGTDSQ PKPDLENRIA EF.

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Specifications

• Antigen: DNA Ligase IV
• Classification: Recombinant Monoclonal
• Concentration: 1.47 mg/mL
• Formulation: PBS with 0.05% BSA, 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P49917
• Gene Symbols: LIG4
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 504-685 of human DNA Ligase IV (P49917).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot
• Clone: 2T6J2
• Conjugate: Unconjugated
• Gene: LIG4
• Gene Alias: 5830471N16Rik; DNA joinase; DNA ligase (ATP) 4; DNA ligase 4; DNA ligase IV; DNA repair enzyme; Lig4; LIG4S; ligase IV, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase; tiny
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3981
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid