Invitrogen™ DNA ligase IV Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA551562

Catalog No. PIPA551562

Description

Immunogen sequence: TYCVIAGSEN IRVKNIILSN KHDVVKPAWL LECFKTKSFV PWQPRFMIHM CPSTKEHFAR EYDCYGDSYF IDTDLNQLKE VFSGIKNSNE QTPEEMASLI ADLEYRYSWD CSPLSMFRRH TVYLDSYA Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 82%.

The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Specifications

• Antigen: DNA ligase IV
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: LIG4
• Gene Alias: 5830471N16Rik; DNA joinase; DNA ligase (ATP) 4; DNA ligase 4; DNA ligase IV; DNA repair enzyme; Lig4; LIG4S; ligase IV, DNA, ATP-dependent; polydeoxyribonucleotide synthase [ATP] 4; polynucleotide ligase; sealase; tiny
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3981
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 0.05 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P49917
• Gene Symbols: LIG4
• Immunogen: Recombinant protein corresponding to Human DNA ligase IV. Recombinant protein control fragment (Product #RP-95411).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG