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Promega DNA Polymerase I

Catalog No. PRM2051 Shop All Promega Corporation Products
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500 U
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PR-M2051 500 U
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Description

DNA Polymerase I catalyzes the template-directed polymerization of nucleotides into duplex DNA in a 5'→3' direction. Recombinant.

  • Catalyzes the Template-Directed Polymerization of Nucleotides into Duplex DNA in a 5′→3′ Direction
  • Can be used in a variety of molecular applications
  • Is inactivated by heating at 68°C for 10 minutes
  • Supplied at a concentration of 5-10u/μL

Specifications

Concentration 5 to 10 U/μL
Color Colorless
Components 10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, DTT 1mM
Purity >90%
pH 7.5
For Use With (Application) Labeling of DNA to high specific radioactivity by nick translation, second-strand cDNA synthesis
Storage Buffer 50mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA, 50% Glycerol
Content And Storage -30°C to -10°C
Source Recombinant E. coli strain
Quantity 500 U
Product Type DNA Polymerase I
Form Liquid
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