Invitrogen™ DNAI1 Monoclonal Antibody (70.1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA122146

Catalog No. MA122146

Description

Recommended positive controls: chicken brain. This antibody is know to detect cytoplasmic Dynein intermediate chains, and reactivity with the axonemal form is not known. By Western blot, MA1-22146 detects a ~ 70 kDa triplet representing the Dynein intermediate chains. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Eukaryotic cells rely on actin and microtubule-based protein 'motors' to generate intracellular movements. These protein 'motors' contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kDa), three intermediate chains (70-125 kDa), and at least four light chains (15-25 kDa).

Specifications

• Antigen: DNAI1
• Classification: Monoclonal
• Concentration: 0.9 mg/mL
• Formulation: Ascites with 15 mM sodium azide
• Gene Accession No.: Q5XIL8, Q9UI46
• Gene Symbols: DNAI1
• Immunogen: Cytoplasmic, full length, native, chicken dynein intermediate chain protein purified from chick brain.
• Regulatory Status: RUO
• Gene ID (Entrez): 100754021, 27019, 431654, 500442
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunomicroscopy, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 70.1
• Conjugate: Unconjugated
• Gene: DNAI1
• Gene Alias: 1110066F04Rik; axonemal dynein intermediate chain 1; b2b1526Clo; BB124644; CILD1; DIC1; Dnai1; Dnaic1; dynein axonemal intermediate chain 1; Dynein intermediate chain 1, axonemal; dynein, axonemal, intermediate chain 1; dynein, axonemal, intermediate polypeptide 1; I79_025143; ICS; ICS1; immotile cilia syndrome 1; MGC26204; PCD; RP11-296L22.2; testis tissue sperm-binding protein Li 87P
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Hamster, Chicken, Amphibian, Marsupial, Deer
• Product Type: Antibody
• Isotype: IgM

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov