Invitrogen™ DNAJB14 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA557494

Catalog No. PIPA557494

Description

Immunogen sequence: RKPSGSGDQS KPNCTKDSTS GSGEGGKGYT KDQVDGVLSI NKCKNYYEVL GVTKDAGDED Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 85%.

Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway (PubMed:24732912). Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities (PubMed:24732912). Can also act independently of HSPA8/Hsc70: together with DNAJB12, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers (PubMed:27916661). While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70 (PubMed:27916661). When overexpressed, forms membranous structures together with DNAJB12 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear (PubMed:24732912). [UniProt].

Specifications

• Antigen: DNAJB14
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Dnajb14
• Gene Alias: 5730496F10Rik; DnaJ (Hsp40) homolog, subfamily B, member 14; DnaJ heat shock protein family (Hsp40) member B14; dnaJ homolog subfamily B member 14; Dnajb14; EGNR9427; PRO34683; UNQ9427/PRO34683
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 499716, 70604, 79982
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q149L6, Q8TBM8
• Gene Symbols: Dnajb14
• Immunogen: Recombinant protein corresponding to Human DNAJB14. Recombinant protein control fragment (Product #RP-96715).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG