Invitrogen™ DNase I Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5122030

Catalog No. PIPA5122030

Description

DNase I Polyclonal Antibody for Western Blot, IHC (P)

Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.

Specifications

• Antigen: DNase I
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DNASE1
• Gene Alias: AI788650; deoxyribonuclease; Deoxyribonuclease 1; deoxyribonuclease I; Deoxyribonuclease1; Deoxyribonuclease-1; DeoxyribonucleaseI; DNASE 1; DNase I; DNase I lysosomal; DNase I, lysosomal; Dnase1; DNaseI; DNL 1; DNL1; Dornase alfa; DRNI; epithelial lens deoxyribonuclease I; FLJ38093; human urine deoxyribonuclease I; pancreatic deoxyribonuclease I
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1773
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300
• Gene Accession No.: P24855
• Gene Symbols: DNASE1
• Immunogen: Recombinant protein Deoxyribonuclease I. The antigen corresponds to amino acid range 19-259 of the target protein.
• Quantity: 20 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG