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Invitrogen™ DNase I Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA576783

Catalog No. PIPA576783


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Description

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

DNase I
Polyclonal
Unconjugated
DNASE1
AI788650; deoxyribonuclease; Deoxyribonuclease 1; deoxyribonuclease I; Deoxyribonuclease1; Deoxyribonuclease-1; DeoxyribonucleaseI; DNASE 1; DNase I; DNase I lysosomal; DNase I, lysosomal; Dnase1; DNaseI; DNL 1; DNL1; Dornase alfa; DRNI; epithelial lens deoxyribonuclease I; FLJ38093; human urine deoxyribonuclease I; pancreatic deoxyribonuclease I
Rabbit
Antigen affinity chromatography
RUO
13419, 1773, 25633
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Liquid
Western Blot
1 mg/mL
PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
P21704, P24855, P49183
DNASE1
Recombinant full length Human DNase I.
100 μL
Primary
Human, Mouse, Rat
Antibody
IgG
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