Invitrogen™ DNMT3A Monoclonal Antibody (64B814)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA191490

Catalog No. PIMA191490

Description

MA1-91490 detects Dnmt3a from mouse samples.

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Specifications

• Antigen: DNMT3A
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% BSA and 0.05% sodium azide
• Gene Accession No.: O88508, Q9Y6K1
• Gene Symbols: DNMT3A
• Immunogen: Bacteria expressed HIS-tag recombinant mouse Dnmt3a.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: 64B814
• Conjugate: Unconjugated
• Gene: DNMT3A
• Gene Alias: AU015806; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3 alpha; DNA methyltransferase 3A; DNA methyltransferase HsaIIIA; DNA methyltransferase MmuIIIA; DNA MTase HsaIIIA; DNA MTase MmuIIIA; Dnmt3a; DNMT3A2; M.HsaIIIA; MmuIIIA; TBRS; W91664
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 13435, 1788
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid