Invitrogen™ DNMT3L Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA599042

Catalog No. PIPA599042

Description

DNMT3L Polyclonal Antibody for Western Blot, ICC/IF, ELISA

Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and development. Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT3L is a catalytically inactive regulatory factor for the DNMT3A and DNMT3B de novo methyltransferases that is expressed at low levels in embryonic stem cells, testis, ovaries, and thymus. These de novo methyltransferases consist of a heterotetrameric complex containing two molecules of DNMT3L, and either two molecules of DNMT3A or DNMT3B. DNMT3L contains an amino-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a carboxy-terminal methyltransferase-like domain.

Specifications

• Antigen: DNMT3L
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DNMT3L
• Gene Alias: cytosine-5-methyltransferase 3-like protein; D6Ertd14e; DNA (cytosine-5-)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; DNA methyltransferase 3 like; Dnmt 3L; DNMT3L; ecat7; human cytosine-5-methyltransferase 3-like protein; MGC1090
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 29947
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 5 mg/mL
• Formulation: PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
• Gene Accession No.: Q9UJW3
• Gene Symbols: DNMT3L
• Immunogen: Recombinant Human DNA (cytosine-5)-methyltransferase 3-like protein (1-234AA).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG