Invitrogen™ DOK6 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536322

Catalog No. PIPA536322

Description

This antibody detects endogenous protein at a molecular weight of 43 kDa. Purity is >95% by SDS-PAGE.

DOK-6 (docking protein 6), is a novel member of the Dok-4/5 subclass of the p62 Dok family of intracellular adaptor molecules. Dok-6 is highly expressed in the developing central nervous system and is co-expressed with Ret in several locations, including sympathetic, sensory, and parasympathetic ganglia, as well as in the ureteric buds of the developing kidneys. Pull-down assays using the Dok-6 phosphotyrosine binding (PTB) domain and GDNF-activated Ret indicate that Dok-6 binds to the phosphorylated Ret Tyr(1062) residue. Moreover, ligand activation of Ret resulted in phosphorylation of tyrosine residue(s) located within the unique C terminus of Dok-6 predominantly through a Src-dependent mechanism, indicating that Dok-6 is a substrate of the Ret-Src signaling pathway. (Crowder RJ, Enomoto H, Yang M, Johnson EM Jr, Milbrandt J. Dok-6, a Novel p62 Dok family member, promotes Ret-mediated neurite outgrowth. J Biol Chem. 2004 Oct 1;279(40):42072-81. Epub 2004 Jul 30. It is also known as DOK6, HsT3226, MGC20785 or IRS. 0.

Specifications

• Antigen: DOK6
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Dok6
• Gene Alias: Docking protein 6; DOK5L; Dok6; Dok-6; Downstream of tyrosine kinase 6; downstream of tyrosine kinases 6; HsT3226; RGD1564376
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 220164, 498898, 623279
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q2MHE5, Q6PKX4
• Gene Symbols: Dok6
• Immunogen: Synthetic peptide corresponding to amino acids 98-149 of human Dok-6.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG