Invitrogen™ Dot1L Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA582437

Catalog No. PIPA582437

Description

Immunogen sequence: LLKARCEELQ LDWATLSLEK LLKEKQALKS QISEKQRHCL ELQISIVELE KSQRQQELLQ LKSCVPPDDA LSLHLRGKGA LGRELEPDAS RLHLELDCTK FSLPHLSSMS PELSMNGQAA GYELCGVLSR PSSKQNTPQY LASPLDQ.

DOT1-like histone lysine methyltransferase (DOT1L) is a gene located on chromosome 19q13.12, encoding an enzyme responsible for methylating lysine 79 on histone H3, a critical modification influencing chromatin structure and gene expression. Unlike most histone methyltransferases, DOT1L does not contain a SET domain and uniquely catalyzes H3K79 methylation, which plays pivotal roles in the regulation of transcription and maintenance of genomic stability. Expression of DOT1L is vital for various biological processes, including cell cycle progression, development, and differentiation, particularly in hematopoietic cells. Aberrations in DOT1L activity have been associated with mixed-lineage leukemia (MLL), where DOT1L-mediated H3K79 methylation contributes to leukemogenesis by influencing gene expression related to proliferation and survival. Due to its involvement in cancer progression, DOT1L is being investigated as a therapeutic target, with inhibitors developed to specifically modulate its enzymatic activity, offering potential for treating MLL and other malignancies.

Specifications

• Antigen: Dot1L
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Dot1l
• Gene Alias: A630076O07; AW907654; DKFZp586P1823; DOT1; DOT1 like histone H3K79 methyltransferase; DOT1 like histone lysine methyltransferase; DOT1L; DOT1-like histone H3 methyltransferase; DOT1-like histone H3K79 methyltransferase; DOT1-like protein; DOT1-like, histone H3 methyltransferase; DOT1-like, histone H3 methyltransferase (S. cerevisiae); H3-K79-HMTase; histone H3 methyltransferase DOT1; histone H3-K79 methyltransferase; histone methyltransferase DOT1L; histone-lysine N-methyltransferase, H3 lysine-79 specific; KIAA1814; KMT4; lysine N-methyltransferase 4; mDot1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 84444
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q8TEK3
• Gene Symbols: Dot1l
• Immunogen: Recombinant protein corresponding to Human DOT1L. Recombinant protein control fragment (Product #RP-105721).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov