Invitrogen™ Doublecortin Monoclonal Antibody (2G5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA517066

Catalog No. PIMA517066

Description

MA5-17066 targets DCX in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human samples. The MA5-17066 immunogen is purified recombinant fragment of human DCX (amino acids: 362-411) expressed in E. Coli. MA5-17066 detects DCX which has a predicted molecular weight of approximately 49.3kDa.

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.

Specifications

• Antigen: Doublecortin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: O43602, O88809
• Gene Symbols: DCX
• Immunogen: Purified recombinant fragment of human DCX (Uniprot: aa 281-335) expressed in E. Coli (NP_000546: aa 462-411).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 2G5
• Conjugate: Unconjugated
• Gene: DCX
• Gene Alias: DBCN; Dbct; DC; Dcn; DCX; doublecortex; doublecortin; doublin; lissencephalin-X; lissencephaly, X-linked (doublecortin); LISX; Lis-X; LIS-XD; Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316; SCLH; XLIS
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 13193, 1641
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid