Thermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM)

Description

Stain live or fixed cells with this far-red DNA stain.

Stain live or fixed cells with Thermo Scientific™ DRAQ5 Fluorescent Probe, a far-red DNA stain.

Fluorescence cell-based assays, such as flow cytometry, in-cell ELISA, fluorescence microscopy and high-content imaging require a fluorescent label to identify individual cells. When using multiple fluorescent probes to detect different cellular targets or activities, each probe must have a fluorescent spectrum different than the other probes. The blue-fluorescent DNA-binding probes, Hoechst and DAPI are frequently used; however, these probes cannot be used when UV illumination is unavailable or other blue-emitting fluorescent probes are used. Therefore, nuclear probes that emit in a color other than blue is useful for cell identification and counting, and for determining nuclear morphology and DNA content.

• Due to its far-red excitation and emission, the DRAQ5 Fluorescent Probe can be multiplexed with many other fluorophores and is ideal for cells expressing green fluorescent protein (GFP) fusion proteins
• Compatible with many existing protocols across a wide range of instrumentation platforms
• Emits in the far-red region, is lipophilic and crosses cell and nuclear membranes in live and fixed cells and tissues for rapid DNA staining
• Stain is water-soluble, supplied ready-to-use and does not require RNase, cell lysis, or a washing step, making it compatible with automation
• Because DNA staining is stoichiometric, the DRAQ5 Fluorescent Probe can be used for DNA content analysis in cell proliferation studies

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Specifications

• Cell Permeability: Cell-permeant
• Color: Red
• Content And Storage: Store at 4°C.
• Description: DRAQ5 Fluorescent Probe Solution (5 mM)
• Detection Method: Fluorescence
• For Use With (Application): Cell Imaging
• For Use With (Equipment): Fluorescence Microscope, High Content Analysis Instrument
• Product Type: Fluorescent Probe Solution
• Dye Type: Cell-Permeant
• Emission: 681
• Excitation Wavelength Range: 647 nm
• Form: Liquid
• Product Line: Pierce
• Purity or Quality Grade: > 99%
• Quantity: 200 μL
• Solubility: Soluble at working concentrations in water and typical buffers
• Sub Cellular Localization: Nucleus, Cytosol, Cytoplasm

For Research Use Only. Not for use in diagnostic procedures.