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Thermo Scientific™ DreamTaq Green DNA Polymerase Available on GSA/VA Contract for Federal Government customers only.

Save time in standard PCR applications with this enhanced Taq DNA polymerase, supplied with colored buffer enabling direct gel loading.

Manufacturer:  Thermo Scientific™ EP0712

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Catalog No. FEREP0712


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Description

Description

DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. DreamTaq DNA Polymerase is an enhanced Taq polymerase optimized for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.

The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. For applications requiring PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless 10X DreamTaq Buffer (#B65) or purifying the PCR product before analysis. The 10X DreamTaq Green Buffer is optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM.

DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.

Highlights

  • Direct loading of PCR product on gel
  • High yields and high sensitivity of PCR
  • Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
  • Robust amplification with minimal optimization
  • Incorporates modified nucleotides, but does not incorporate dUTP

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Specifications

Specifications

Routine PCR amplification of DNA fragments up to 6kb; RT-PCR; Genotyping; Generation of PCR products for TA cloning
2 x 1.25mL of 10X DreamTaq Green Buffer
DreamTaq Green DNA Polymerase
500U (5U/µL)
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This product is licensed under one or more U.S. Patents Nos. 5,500,363 and 5,352,778 or corresponding foreign patents. This product is licensed under one U.S. Patent No. 5,436,149 or corresponding foreign patents owned by Takara Bio, Inc.

Patent pending.