Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format

Description

Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.

Thermo Scientific™ Pierce DSS, also called disuccinimidyl suberate, is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

Highlights:

• Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
• “Fix” protein interactions to allow identification of weak or transient protein interactions
• Protein crosslinking to create bioconjugates via single-step reactions
• Crosslink oriented antibodies to Protein A or G agarose
• Immobilize proteins onto amine-coated surfaces

Specifications

• Chemical Reactivity: Amine-Amine
• Cleavable: No
• Description: DSS
• Molecular Weight (g/mol): 368.35
• PEGylated: No
• Spacer Arm Length: 11.4 Å
• Content And Storage: Upon receipt store desiccated at 4°C.
• Cell Permeability: Yes
• Shipping Condition: Ambient
• Product Line: Pierce™
• Labeling Method: Chemical Labeling
• Crosslinker Type: Homobifunctional
• Reactive Moiety: NHS Ester
• Spacer: Medium (10 to 30 Å)
• Form: Powder
• Quantity: 1 g
• Solubility: DMF, DMSO
• Format: Standard, Single-use
• Water Soluble: No
• Product Type: Crosslinker

For Research Use Only. Not for use in diagnostic procedures.