Invitrogen™ DTL Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA542842

Catalog No. PIPA542842

Description

Peptide sequence: VNQISGAHNT SDKQTPSKPK KKQNSKGLAP SVDFQQSVTV VLFQDENTLV Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 86%; Rat: 100%.

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Specifications

• Antigen: DTL
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DTL
• Gene Alias: 2810047L02Rik; 5730564G15Rik; CDT2; CDW1; DCAF2; DDB1 and CUL4 associated factor 2; DDB1- and CUL4-associated factor 2; denticleless E3 ubiquitin protein ligase homolog; denticleless E3 ubiquitin protein ligase homolog (Drosophila); denticleless homolog; denticleless homolog (Drosophila); denticleless protein homolog; DTL; L2dtl; lethal(2) denticleless protein homolog; Meth A RAMP; Meth A retinoic acid-regulated nuclear matrix-associated protein; RA regulated nuclear matrix associated protein; RAMP; RA-regulated nuclear matrix-associated protein; Retinoic acid-regulated nuclear matrix-associated protein; RGD1310439
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 51514
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 2% sucrose and 0.09% sodium azide
• Gene Accession No.: Q9NZJ0
• Gene Symbols: DTL
• Immunogen: Synthetic peptide directed towards the N-terminal of human DTL (aa 182-231).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov