Invitrogen™ DUX4 Monoclonal Antibody (P2B1), PerCP

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545267

Catalog No. PIMA545267

Description

1 μg/mL of MA5-45267 was sufficient for detection of DUX4 in 20 μg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary. Detects approximately 45kDa. No cross-reactivity with DUX4c.

DUX4 (also known as Double homeobox 4) is the leading candidate causative gene for facioscapulohumeral dystrophy (FSHD), a degenerative skeletal muscle disease and one of the most common muscular dystrophies. FSHD is caused by the deletion of a subset of D4Z4 macrosatellite repeats on chromosome 4. Each repeat contains a retrogene encoding the double-homeobox factor DUX4. DUX4 expression is epigenetically suppressed in differentiated tissues and the residual DUX4 transcripts are spliced to remove the carboxyterminal domain that has been associated with cell toxicity. In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle, and possibly other differentiated tissues, and results in a small percentage of cells expressing relatively abundant amounts of the full-length DUX4 mRNA and protein.

Specifications

• Antigen: DUX4
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: Q9UBX2
• Gene Symbols: Dux4
• Immunogen: C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Clone: P2B1
• Conjugate: PerCP
• Gene: Dux4
• Gene Alias: AW822073; double homeobox; double homeobox 4; double homeobox 4-like; double homeobox B-like 1; double homeobox protein 10; Double homeobox protein 4; double homeobox protein 4/10; Dux; DUX10; Dux4; DUX4L; Duxbl; Duxbl1; EG664783; homeobox protein; RGD1311053
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100288687
• Content And Storage: 4°C
• Form: Liquid