DYKDDDDK Monoclonal Antibody (29E4.G7), Rockland™

Catalog No.	Quantity
50-199-0524	25 μL
50-199-0523	100 μg
50-194-3794	500 μg

Catalog No. 50-199-0524 Supplier Rockland Immunochemicals Supplier No. 200301383S

Description

Mouse Monoclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. The purified antibody is directed against the FLAGTM motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAGTM tag antibody detects over-expressed proteins containing the FLAGTM epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

DYKDDDDK Tag (FLAG™ tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. FLAG is a trademark of Sigma-Aldrich Biotechnology LP

Specifications

• Antigen: DYKDDDDK
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 29E4.G7
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
• Host Species: Mouse
• Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAGTM epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
• Quantity: 25 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ
• Target Molecule: Tag