Ambion Dynabeads mRNA DIRECT Purification Kit
Manufacturer: Ambion 61011
DescriptionThe Dynabeads mRNA DIRECT Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications. Advantages of the Dynabeads mRNA DIRECT Kit:
The isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads . Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. The mRNA purification beads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples (see protocol). 1 mg of Dynabeads Oligo (dT)25 beads (200µL) binds up to 2µg of mRNA. A typical mammalian cell contains about 1030 pg of total RNA, from which 15% is mRNA.
|Not High-throughput Compatible (Manual)|
|DYNAL®, Dynabeads®, mRNA DIRECT™|
|Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Nuclease Protection Assays, Northern Blotting, Cloning|
|Liquid Samples (e.g. Serum), Plant Samples, Viral Samples, Cells, Tissue, FFPE & Fixed Samples, Yeast, RNA, Blood|
5 ml Dynabeads oligo (dT)25
30 ml Lysis/Binding Buffer
60 ml Washing Buffer A
30 ml Washing Buffer B
15 ml 10 mM Tris-HCl (Elution buffer)
Contains enough reagents for 20 standard isolations. Store at 2 to 8°C.
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