Invitrogen™ Dynactin 1 Monoclonal Antibody (6B11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA518308

Catalog No. PIMA518308

Description

This antibody detects endogenous levels of DCTN1 at a molecular weight of 150 kDa and does not cross-react with related proteins. Purity is >95% by SDS-PAGE.

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).

Specifications

• Antigen: Dynactin 1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q14203
• Gene Symbols: DCTN1
• Immunogen: Synthetic peptide corresponding to the N-terminal of human Dynactin 1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Immunoprecipitation, Western Blot
• Clone: 6B11
• Conjugate: Unconjugated
• Gene: DCTN1
• Gene Alias: 150 kDa dynein-associated polypeptide; AL022633; DAP 150; DAP150; DAP-150; DCTN 1; DCTN1; DP 150; DP150; DP-150; dynactin 1; dynactin 1 (p150, glued homolog, Drosophila); dynactin 1, retrograde axonal transport; dynactin subunit 1; Dynactin1; Glued; HMN7B; OTTHUMP00000202491; p135; p150; p150 glued; p150 glued homolog; p150>glued>; p150glued; p150-glued
• Host Species: Mouse
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1639
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid