Invitrogen™ Dynactin 2 Polyclonal Antibody

Sheep Polyclonal Antibody

Supplier:  Invitrogen™ PA547881

Catalog No. PIPA547881

Description

This antibody detects human Dynactin Subunit 2/DCTN2 in direct ELISAs and human and mouse Dynactin Subunit 2/DCTN2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DCTN1 is observed. Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kDa. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Specifications

• Antigen: Dynactin 2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DCTN2
• Gene Alias: 2310042E05Rik; 50 kDa dynein-associated polypeptide; C130077D06Rik; C87049; DCTN2; dctn2 protein; DCTN50; DCTN-50; dynactin 2; dynactin 2 (p50); dynactin complex 50 kDa subunit; Dynactin subunit 2; dynamitin; epididymis secretory protein Li 77; GMP23-48K; Growth cone membrane protein 23-48K; HEL-S-77; hypothetical protein LOC527201; p50; p50 dynamitin; p50/dynamitin; RBP50; zgc:63867
• Host Species: Sheep
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10540, 69654
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Western Blot, Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q13561, Q99KJ8
• Gene Symbols: DCTN2
• Immunogen: E. coli-derived recombinant human Dynactin Subunit 2/DCTN2 Asp277-Lys401.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG