Thermo Scientific™ DyNAmo HS SYBR Green qPCR Kit

Catalog No. F410L

Supplier: Thermo Scientific™ F410L

Description

Contains a modified Thermus brockianus DNA polymerase and all the other reagents needed for qPCR.

Only template DNA and PCR primers need to be added by the user. The modified DNA polymerase in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. ROX passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.

• Strong specificity: DyNAmo HS SYBR Green master mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers during reaction setup
• Highly sensitive detection: Optimized master mix allows reproducible detection of low-copy number templates
• Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude
• Convenient to use: 2X master mix, one tube only; ROX passive reference dye provided in a separate vial

Specifications

• Concentration: 2X (qPCR Mix), 50X (Dye)
• Content And Storage: Contains:
  • 5 x1 mL DyNAmo HS SYBR Green qPCR Mix (2X)
  • 250 μL 50X ROX dye, 25μM
  
  Store at -20°C
• Detection Method: SYBR
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: Tbr DNA polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
• Kit Type: DyNAmo HS SYBR Green qPCR Kit
• Product Line: DyNAmo
• Product Type: Real Time PCR SYBR Master Mix
• Quantity: 500 reactions
• Sufficient For: 500 Reactions of 20 μL Each, 200 Reactions of 50 μL Each
• For Use With (Application): Gene Expression
• No. of Reactions: 500 Reactions
• Sample Type: DNA, dsDNA
• Volume: 5 x 1 mL

For Research Use Only. Not for use in diagnostic procedures.