MilliporeSigma™ Dynein, HC, cytosolic, Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABT266MI	100 μg	Unconjugated

Catalog No. ABT266MI Supplier MilliporeSigma™ Supplier No. ABT266

Description

Rabbit Polyclonal Antibody

Cytoplasmic dynein 1 heavy chain 1 (UniProt Q14204; also known as Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic) is encoded by the DYNC1H1 (also known as CMT2O, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325, SMALED1) gene (Gene ID 1778) in human. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. Cytoplasmic dynein 1 is a multisubunit complex of ∽1.5 MDa composed of a homodimer of heavy chains (encoded by DYNC1H1), two intermediate chains (encoded by DYNC1I1 and DYNC1I2), light-intermediate chains (encoded by DYNC1LI1, DYNC1LI2) and light chains (encoded by DYNLT1, DYNLT3, DYNLRB1, DYNLRB2, DYNLL1, DYNLLl2). The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. The intermediate chain proteins (IC1 and IC2) are involved in cargo binding and specificity, either alone or via interaction with the dynactin complex. IC1 and IC2 share 69% protein identity. They interact with the dynein light chains and the p150 subunit of dynactin at the N-terminus and with the heavy chains through WD40 repeats at the intermediate chain C-terminus.

Specifications

• Antigen: Dynein, HC, cytosolic
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: DYNC1H1, CMT2O, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325, SMALED1
• Gene Accession No.: Q14204
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to a sequence within the second AAA domain of human cytoplasmic dynein heavy chain.
• Purification Method: Affinity Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001367
• Test Specificity: This polyclonal antibody targets a sequence within the second AAA domain of cytoplasmic dynein heavy chain encoded by the DYNC1H1 gene.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

For Research Use Only. Not for use in diagnostic procedures.