MilliporeSigma™ DYRK1A, Mouse, Unlabeled, Clone: 7F3,

Catalog No.	Quantity	Conjugate
MABN1835MI	100 μg	Unconjugated

Catalog No. MABN1835MI Supplier MilliporeSigma™ Supplier No. MABN1835

Description

Mouse Monoclonal Antibody

Dual specificity tyrosine-phosphorylation-regulated kinase 1A (EC 2.7.12.2; UniProt Q63470; also known as Dual specificity YAK1-related kinase, MNBH, Protein kinase minibrain homolog, RP86) is encoded by the Dyrk1a (also known as Dyrk) gene (Gene ID 25255) in rat species. DYRK1A belongs to a family of conserved dual-specificity tyrosine-phosphorylated and regulated kinases (DYRKs) within the CMGC (CDK, MAPK, GSK, and CLK) group of eukaryote kinome. DYRK family members share a conserved kinase domain and an adjacent DYRK-homology domain or DH-box (DDDNXDY), wihile differing in their N- and C-terminal regions. DYRK1A is regulated by autophosphorylation on Ser, Thr and Tyr residues, but catalyzes phosphorylation of a broad-range of substrates only on Ser/Thr residues within the RPX(S/T)P motif with a preference for Pro at the +1 position (X). Known DYRK1A substrates include transcription factors (e.g. FKHR, CREB, Gli1, NFAT), splicing factors (e.g. cycling L2, SF3b/SAP155), glycogen synthase, as well as multiple proteins engaged in endocytosis in neurons, including dynamin and synaptojanin 1. DYRK1A is also implicated in promoting the formation of neurotoxic Aβ peptides by phosphorylating APP and presenilin-1. Human DYRK1A gene is located in the Down syndrome critical region of chromosome 21 and the protein plays an essential role in the development of the central nervous system. DYRK1A haploinsufficiency and mutations are the causes of intellectual disability (ID), microcephaly and dysmorphic features in human, while transgenic mice carrying extra copies of the gene exhibit learning defects and motor abnormalities.

Specifications

• Antigen: DYRK1A
• Applications: Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 7F3
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse IgG2bκ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: Dyrk1a, Dyrk
• Gene Accession No.: Q63470
• Host Species: Mouse
• Immunogen: His-tagged recombinant rat DYRK1A N-terminal fragment (Wegiel, J., et al. (2004). Brain Res. 1010(1-2):69-80).
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_036923
• Test Specificity: The specificity of target band detection was confirmed by antibody blocking with the immunogen prior to Western blotting (Wegiel, J., et al. (2004). Brain Res. 1010(1-2):69-80).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only. Not for use in diagnostic procedures.