Invitrogen™ Dysadherin Monoclonal Antibody (M029)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547667

Catalog No. PIMA547667

Description

Clone M029 mouse monoclonal antibody detects a 35-55 kDa* band on SDS-PAGE immunoblots of human A431, A549, MeWo, MDA-MB-231 cell, as well as human breast, lung, and skin tissues. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the FXYD5 protein on live cells.

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 in variant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, is a glycoprotein that functions in the up-regulation of chemokine production, and it is involved in the reduction of cell adhesion via its ability to down-regulate E-cadherin. It also promotes metastasis, and has been linked to a variety of cancers. Alternative splicing results in multiple transcript variants.

Specifications

• Antigen: Dysadherin
• Classification: Monoclonal
• Concentration: 0.25 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q96DB9
• Gene Symbols: FXYD5
• Immunogen: Clone (M029) was generated from a proprietary antigen related to the native human FXYD5 protein expressed in A431 epidermoid carcinoma cell line.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: M029
• Conjugate: Unconjugated
• Gene: FXYD5
• Gene Alias: DYSAD; Dysadherin; EF-8; FXYD domain containing ion transport regulator 5; FXYD domain-containing ion transport regulator 5; FXYD5; HSPC113; Ion channel homolog RIC; IWU1; IWU-1; KCT1; keratinocytes associated transmembrane protein 1; OIT2; oncoprotein induced transcript 2; oncoprotein-induced protein 2; PRO6241; RIC; UNQ2561/PRO6241
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 53827
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid