Invitrogen™ E-Cadherin Monoclonal Antibody (4A2C7)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 180223

Catalog No. 18-022-3

Description

This monoclonal antibody reacts with human epithelial cadherin (E-cadherin, uvomorulin, Cell-CAM 120/80), a 120 kDa transmembrane glycoprotein. Reduction and/or loss of E-cadherin expression in carcinomas correlates positively with tumor cell invasion and metastasis. Altered expression of E-cadherin is common in the progression of cancers including breast carcinoma, lung carcinoma, gastric tumors, hepatocellular carcinoma, and prostatic tumors. It has recently been reported that E-cadherin is a useful marker for differentiating lobular lesions (negative) from ductal invasive lesions (positive) in breast cancer. Positive tissue: Non-neural epithelia. Reacts strongly with human E-cadherin and weakly with human P-cadherin. Expected Staining Pattern: Mainly cellular membrane, some cytoplasmic.

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

Specifications

• Antigen: E-Cadherin
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA and 0.1% sodium azide
• Gene Accession No.: P12830
• Gene Symbols: CDH1
• Immunogen: Recombinant protein corresponding to the cytoplasmic domain of human E-cadherin.
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 999
• Test Specificity: E-Cadherin
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ
• Reactivity: Human
• Reactivity abrv: Hu
• Validated Application abrv: IHC (FFPE)

• Applications: Immunohistochemistry (Paraffin)
• Clone: 4A2C7
• Dilution: Concentrate Antibody
• Gene: CDH1
• Gene Alias: AA960649; ARC-1; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, epithelial; cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell adhesion molecule; cell-CAM 120/80; ECAD; E-cad; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Ecadherin; E-cadherin; E-cadherin 1; E-cadherin precursor; Epithelial cadherin; hab; half baked; LCAM; L-CAM; Um; UVO; Uvomorulin
• Host Species: Mouse
• Positive Control: Non-neural epithelia
• Quantity: 1 mL
• Primary or Secondary: Primary
• Packaging: 1 Each
• Includes: 0.1% Sodium azide
• Content And Storage: 2-8°C
• Form: Liquid
• Host abrv: Ms
• Shipping Condition: Wet Ice
• Target Gene: E-Cadherin