MilliporeSigma™ E. coli anti-poly-ADP-ribose binding reagent, Polyclonal,

E. coli Polyclonal Antibody

Supplier:  MilliporeSigma™ MABE1031

Catalog No. MABE1031MI

Description

Specifically detects poly-ADP-ribose binding reagent in Human, Mouse samples, and it is validated for Dot Blot

Anti-poly-ADP-ribose binding reagent, is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta™(DE3)pLysS strain of E. coli. Anti-poly-ADP-ribose binding reagent is useful for the affinity detection of oligo- and poly-ADP-ribosylated (PARylated) proteins on membranes or on fixed cells in a manner similar to antibody-based Western blot, dot blot, and immunocytochemistry applications. The rabbit Fc tag allows visualization of the binding/labeling with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-poly-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.

Specifications

• Antigen: poly-ADP-ribose binding reagent
• Classification: Polyclonal
• Host Species: E. coli
• Quantity: 200 μL
• Research Discipline: Epigenetics and Nuclear Function
• Target Species: Human, Mouse
• Form: Purified

• Applications: Dot Blot
• Formulation: Purified from E. coli by Ni-NTA agarose. Supplied in buffer containing 10mM Tris pH 7.5, 0.2M NaCl, 10% Glycerol, 10mM Imidazole, 1mM PMSF, 1mM β-Mercaptoethanol, 10% glycerol without preservatives.
• Purification Method: Ni-NTA agarose
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Stable for one year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.

For Research Use Only