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E.coli Combined HCP Rabbit anti-Bacteria, Biotin, Polyclonal, Rockland™
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Catalog No. 501990997
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100 μg
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Catalog No. Quantity
50-199-0997 100 μg
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Catalog No. 50-199-0997 Supplier Rockland Immunochemicals Supplier No. 200406M61
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Description

Rabbit Polyclonal Antibody

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.

Specifications

Antigen E.coli Combined HCP
Applications ELISA, Immunoprecipitation, Western Blot
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Biotin
Formulation 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
Host Species Rabbit
Immunogen Anti-Host Cell Proteins Antibody was prepared by repeated immunizations with a full range of high molecular weight (HMW) and low molecular weight (LMW) proteins from E.Coli (BL21).
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Bacteria
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Lyophilized
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