Invitrogen™ E-selectin Recombinant Rabbit Monoclonal Antibody (103)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA529553

Catalog No. PIMA529553

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human E-Selectin/CD62e.

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Specifications

• Antigen: E-selectin
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P16581
• Gene Symbols: SELE
• Immunogen: Recombinant Human E-Selectin/CD62e protein (Met1-Pro556).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Flow Cytometry, Western Blot
• Clone: 103
• Conjugate: Unconjugated
• Gene: SELE
• Gene Alias: CD62 antigen-like family member E; CD62E; e selectin; Elam; ELAM1; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule; endothelial leukocyte adhesion molecule 1; endothelial leukocyte adhesion molecule I; ESEL; E-selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; SELE; selectin E; selectin E (endothelial adhesion molecule 1); selectin, endothelial cell
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 6401
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid